Date of Award
Spring 2020
Thesis Type
Open Access
Degree Name
Honors Bachelor of Arts
Department
Biology
Sponsor
Dr. Jay Pieczynski
Committee Member
Dr. Brendaliz Santiago-Narvaez
Committee Member
Dr. Pamela Brannock
Abstract
Our nervous system propagates signals by releasing neurotransmitters into the synaptic cleft between two adjacent neurons. The transport and delivery of such neurotransmitters within the neuron is reliant on microtubule structure and motor proteins present. The direction of motor protein transport is dictated by the inherent polarity of microtubules. Kinesin-3 motor proteins, such as KLP-4 found in Caenorhabditis elegans, use this polarity to travel in an anterograde direction towards neurotransmitter exocytosis sites. Thus, KLP-4 is associated with the maintenance of neuron functionality. The klp-4 (ok3537) allele results in a large, in frame deletion within the cargo-binding domain of the motor protein, causing unknown molecular consequences. C. elegans containing this allele experience difficulty moving in reverse and disorganized synapses. Cargo that binds to the KLP-4 cargo-binding region is unknown. By tagging both wild type and mutant KLP-4 with a Myc tag, we planned to isolate and identify the proteins bound to the cargo binding domain. Additionally, in the presence of mutant KLP-4, the microtubules it travels on experience an increase in acetylation. Microtubule acetylation stabilizes microtubules and helps motor proteins travel efficiently. We suspect the combination of both functional alpha tubulin acetyltransferases, such as MEC-17 and ATAT-2, and mutant KLP-4, which signals for excess microtubule acetylation, results in the disorganized synapses associated with klp-4 (ok3537). We hypothesize that producing an organism homozygous for both the klp-4 (ok3537) allele and a knockout acetyltransferase allele, either mec-17 (ok2109) or atat-2 (ok2415), we would rescue of the mutant KLP-4 phenotype.
Recommended Citation
Wright, Lindsey, "The Physical Interactions of KLP-4 & The Effects of Microtubule Acetylation" (2020). Honors Program Theses. 119.
https://scholarship.rollins.edu/honors/119
Rights Holder
Lindsey Wright